MMP3 ELISA Kit for Human(Homo sapiens) | EIAab.comMMP3 ELISA Kit for Human(Homo sapiens)

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MMP3 (Gene name),

Stromelysin-1 (Protein name ), MMP3_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (2)

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Gene name:

MMP3(STMY1);

Protein name:

Stromelysin-1(SL-1);

Alternative:

Transin-1;Matrix metalloproteinase-3(MMP-3);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Subcellular Location:

Secreted extracellular space extracellular matrix

Protein Attributes:

Sequence length:

477

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

477:

TGSSQLEFDP | NAKKVTHTLK | SNSWLNC

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.375129

MIM:

185250

STRING:

9606.ENSP00000299855

SMR:

P08254

Pfam:

PF00045

String:

P08254

KEGG:

hsa:4314

Uniprot:

P08254

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ELISA Kit for Human SL-1

Cat.:

E0101h

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96T

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ELISA Kit for Human SL-1

Cat.:

E0101m

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96T

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ELISA Kit for Human SL-1

Cat.:

E0101r

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96T

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ELISA Kit for Human SL-1

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ELISA Kit for Human SL-1

Cat.:

E0101d

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96T

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CLIA Kit for Human SL-1

Cat.:

U0101d

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96T

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CLIA Kit for Human SL-1

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CLIA Kit for Human SL-1

Cat.:

U0101r

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human SL-1

Cat.:

U0101h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

Please sign in first.

CLIA Kit for Human SL-1

Cat.:

U0101m

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Looking for manual.

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96T

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Polyclonal Antibody for Human SL-1

Cat.:

P0101Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human SL-1

Cat.:

P0101Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human SL-1

Cat.:

P0101Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human SL-1

Protein for Human SL-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of MMP3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	96-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-99%	93-100%	95-102%	98-106%
EDTA plasma(n=5)	96-102%	100-104%	96-103%	97-108%
heparin plasma(n=5)	98-105%	102-109%	97-100%	89-96%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"The protective effect of xanthan gum on interleukin-1β induced rabbit chondrocytes"

G Han, H Shao, X Zhu, et al.

From:Carbohydrate Polymers

[2].

"The clinical implications of increased cyclophilin A levels in patients with acute coronary syndromes"

Jinchuan Yan, , Xuan Zang, Rui Chen, Wei Yuan, Jie Gong, Cuiping Wang, Ying Li

From:Clinica Chimica Acta

References

1.

"The complete primary structure of human matrix metalloproteinase-3. Identity with stromelysin."
Saus J. , Quinones S. , Otani Y. , Nagase H. , Harris E.D. Jr. , Kurkinen M.
J. Biol. Chem.263:6742-6745(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 18-24
2.

"Comparison of human stromelysin and collagenase by cloning and sequence analysis."
Whitham S.E. , Murphy G. , Angel P. , Rahmsdorf H.J. , Smith B. , Lyons A. , Harris T.J.R. , Reynolds J.J. , Herrlich P. , Docherty A.J.P.
Biochem. J.240:913-916(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Fibroblast.
3.

"Human skin fibroblast stromelysin: structure, glycosylation, substrate specificity, and differential expression in normal and tumorigenic cells."
Wilhelm S.M. , Collier I.E. , Kronberger A. , Eisen A.Z. , Marmer B.L. , Grant G.A. , Bauer E.A. , Goldberg G.I.
Proc. Natl. Acad. Sci. U.S.A.84:6725-6729(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Three matrix metalloproteinases on 81kb of P1 insert."
Lin D. , Duncan M. , Allen E. , Araujo R. , Aparicio A. , Chai A. , Chung E. , Davis K. , Federspiel N. , Hyman R. , Kalman S. , Komp C. , Kurdi O. , Lashkari D. , Lew H. , Namath A. , Oefner P. , Roberts D. , more...
Submitted (1996-12) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Synovium.
6.

Suzuki Y. , Sugano S. , Totoki Y. , Toyoda A. , Takeda T. , Sakaki Y. , Tanaka A. , Yokoyama S.
Submitted (2005-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT GLU-45
tissue: Synovium.
7.

SeattleSNPs variation discovery resource
Submitted (2001-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT GLU-45
8.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT GLU-45
tissue: Lung.
10.

"Stepwise activation mechanisms of the precursor of matrix metalloproteinase 3 (stromelysin) by proteinases and (4-aminophenyl)mercuric acetate."
Nagase H. , Enghild J.J. , Suzuki K. , Salvesen G.
Biochemistry29:5783-5789(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ZYMOGEN ACTIVATION
11.

"Progression of coronary atherosclerosis is associated with a common genetic variant of the human stromelysin-1 promoter which results in reduced gene expression."
Ye S. , Eriksson P. , Hamsten A. , Kurkinen M. , Humphries S.E. , Henney A.M.
J. Biol. Chem.271:13055-13060(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN CHDS6
12.

"Prediction of the risk of myocardial infarction from polymorphisms in candidate genes."
Yamada Y. , Izawa H. , Ichihara S. , Takatsu F. , Ishihara H. , Hirayama H. , Sone T. , Tanaka M. , Yokota M.
N. Engl. J. Med.347:1916-1923(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN CHDS6
13.

"The NMR structure of the inhibited catalytic domain of human stromelysin-1."
Gooley P.R. , O'Connell J.F. , Marcy A.I. , Cuca G.C. , Salowe S.P. , Bush B.L. , Hermes J.D. , Esser C.K. , Hagmann W.K. , Springer J.P. , Johnson B.A.
Nat. Struct. Biol.1:111-118(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF CATALYTIC DOMAIN
14.

"Solution structures of stromelysin complexed to thiadiazole inhibitors."
Stockman B.J. , Waldon D.J. , Gates J.A. , Scahill T.A. , Kloosterman D.A. , Mizsak S.A. , Jacobsen E.J. , Belonga K.L. , Mitchell M.A. , Mao B. , Petke J.D. , Goodman L. , Powers E.A. , Ledbetter S.R. , Kaytes P.S. , Vogeli G. , Marshall V.P. , Petzold G.L. , more...
Protein Sci.7:2281-2286(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 100-267
15.

"Stromelysin-1: three-dimensional structure of the inhibited catalytic domain and of the C-truncated proenzyme."
Becker J.W. , Marcy A.I. , Rokosz L.L. , Axel M.G. , Burbaum J.J. , Fitzgerald P.M.D. , Cameron P.M. , Esser C.K. , Hagmann W.K. , Hermes J.D. , Springer J.P.
Protein Sci.4:1966-1976(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 18-272
16.

"X-ray structure of a hydroxamate inhibitor complex of stromelysin catalytic domain and its comparison with members of the zinc metalloproteinase superfamily."
Dhanaraj V. , Ye Q.-Z. , Johnson L.L. , Hupe D.J. , Otwine D.F. , Dunbar J.B. Jr. , Rubin J.R. , Pavlovsky A. , Humblet C. , Blundell T.L.
Structure4:375-386(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 100-266
17.

"Inhibition of stromelysin-1 (MMP-3) by P1'-biphenylylethyl carboxyalkyl dipeptides."
Esser C.K. , Bugianesi R.L. , Caldwell C.G. , Chapman K.T. , Durette P.L. , Girotra N.N. , Kopka I.E. , Lanza T.J. , Levorse D.A. , Maccoss M. , Owens K.A. , Ponpipom M.M. , Simeone J.P. , Harrison R.K. , Niedzwiecki L. , Becker J.W. , Marcy A.I. , Axel M.G. , more...
J. Med. Chem.40:1026-1040(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 105-264
18.

"Mechanism of inhibition of the human matrix metalloproteinase stromelysin-1 by TIMP-1."
Gomis-Rueth F.-X. , Maskos K. , Betz M. , Bergner A. , Huber R. , Suzuki K. , Yoshida N. , Nagase H. , Brew K. , Bourenkov G.P. , Bartunik H. , Bode W.
Nature389:77-81(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 100-267 IN COMPLEX WITH TIMP1
19.

"Structural characterizations of nonpeptidic thiadiazole inhibitors of matrix metalloproteinases reveal the basis for stromelysin selectivity."
Finzel B.C. , Baldwin E.T. , Bryant G.L. Jr. , Hess G.F. , Wilks J.W. , Trepod C.M. , Mott J.E. , Marshall V.P. , Petzold G.L. , Poorman R.A. , O'Sullivan T.J. , Schostarez H.J. , Mitchell M.A.
Protein Sci.7:2118-2126(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 100-264
20.

"Crystal structure of the stromelysin catalytic domain at 2.0-A resolution: inhibitor-induced conformational changes."
Chen L. , Rydel T.J. , Gu F. , Dunaway C.M. , Pikul S. , Dunham K.M. , Barnett B.L.
J. Mol. Biol.293:545-557(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 100-272
21.

"X-ray structure of human stromelysin catalytic domain complexed with nonpeptide inhibitors: implications for inhibitor selectivity."
Pavlovsky A.G. , Williams M.G. , Ye Q.-Z. , Ortwine D.F. , Purchase C.F. II , White A.D. , Dhanaraj V. , Roth B.D. , Johnson L.L. , Hupe D. , Humblet C. , Blundell T.L.
Protein Sci.8:1455-1462(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 100-267
22.

"Solution structure of the catalytic domain of human stromelysin-1 complexed to a potent, nonpeptidic inhibitor."
Li Y.C. , Zhang X. , Melton R. , Ganu V. , Gonnella N.C.
Biochemistry37:14048-14056(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 100-272

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